How to Choose the Right Chromatography Solution for Your Bioprocess
Chromatography is a critical step in many biopharma and biotech processes. In our guide, we help you determine the best solution for your specific needs.
What is Chromatography?
Chromatography is a crucial step in bioprocessing, serving as a powerful tool to separate complex mixtures into their individual components. It typically occurs during the early stages of downstream bioprocessing after an initial cell separation and lysate clarification. This separation is essential for both quantifying different compounds within a mixture and for isolating specific molecules for further use, such as a final therapeutic product.
We previously explored how chromatography fits into the greater bioprocess in greater detail in our article “What is Downstream Bioprocessing?”.
Preparative Monolithic Chromatography Columns
Eliminate packing, repacking, and unpacking from your downstream purification process with our Preparative Monolithic Chromatography Columns. Coming in a range of scalable volumes from 1mL to 40L, our columns boast a high viral binding capacity and low shear stress.
The unique housing of the Preparative Monolithic Chromatography Columns forces the liquid inside to follow a radial direction, and its highly cross linked, porous monolithic polymers have a well-defined channel size distribution. Available in single and multi-use, the columns are manufactured with epoxy thermoset materials and reinforced with carbon fibers.
Preparative Monolithic Chromatography Columns are Right for You if You…
- Have applications in downstream purification of large biomolecules, viruses, mRNA, VLPs, pDNA, and exosomes
- Need to accommodate a volume of 1mL to 40L
- Are looking for rapid mass transfer
- Require low back pressure, versatility, and flow-independent performance
- Would like faster concentration, analysis, purification, separation, and removal of biologic materials
Analytical Monolithic Chromatography Columns
Our Analytical Monolithic Chromatography Columns feature flow independent separation, making them ideal for analysis and control of manufacturing processes. Providing reproducible HPLC monitoring, our columns allow you to obtain information about product purity and quantity in a matter of minutes. Operating flow rates of 0.2-3 mL/min are available.
The Analytical Monolithic Chromatography Columns can be used for the identification, separation, and quantitation of biologic and chemical compounds. Ultimately, they have been designed to perfectly combine all characteristics of monoliths into a single process. They are multi-use, and come in a stainless steel housing. Additionally, they can be used alongside multiple detectors.
Analytical Monolithic Chromatography Columns are Right for You if You…
- Have applications across antibody-derived therapeutics, biosimilars, virus, vaccine, plasma proteins, and more
- Require a channel size between 1.3 um and 2 um
- Need a chromatography column that is suitable for analysis of complex samples
- Only have minute amount of your valuable samples, and need high sensitivity to work with them
- Are looking for scalable data for fast method development
PATfix Process Analytical Technology
Ideal for routine gradient separations, our PATfix Analytical HPLC System has a high pressure gradient pump, and enables you to do every analytical task. The HPLC is a highly sensitive multi-wavelength detector, and can follow impurity, product formation, and clearance in upstream and downstream processes.
Aiming to ensure that quality is built into products by design, and enhance bioprocess understanding, our PATfix Analytical HPLC System has a temperature controlled autosampler for maximal working efficiency.
PATfix Process Analytical Technology is Right for You if You…
- Have applications including raw material quality evaluation, raw material control, monitoring of impurity formation, adenovirus purification, and more
- Are looking for extensive safety features like a leak management sensor
- Need additional features in your HPLC system such as a fluorescence detector, and a highly sensitive multi-wavelength detector
- Require pump head flow rates up to 10mL/min
- Would like quick and efficient quantification of key process parameters
Still Not Sure Which Chromatography Solution is Right for You?
Choosing the perfect solutions for your process can be stressful and time consuming. At HPNE, a Getinge Company, we are dedicated to connecting you to the perfect solutions for your unique needs as quickly and efficiently as possible. Our expert team has decades of combined experience pairing each customer with their ideal bioprocessing equipment.
Still not sure what chromatography solution is right for you, or have questions about other bioprocessing equipment like single-use assemblies, pumps, sensors, and more? Drop our team a line at info@hp-ne.com, or visit our contact us form and we will get back to you as soon as we can!
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About HPNE
As the industry needs grow, High Purity New England, Inc. continues to supply the biopharmaceutical industry with a range of innovative products, from drug discovery and development to fill-finish, including their flagship product, custom single-use assemblies, as well as pumps, sensors, bioreactor systems, storage and handling solutions and other single-use solutions. Along with their own manufactured products for the global market, they are also a distributor for more than 18 brands in North America.
